Isolation of Diarrheagenic Escherichia coli and their Specific Phages from Rabbits

This study designed to isolation virulence E. coli, specific phages and identify in rabbits biochemically, serologically, detect genes using PCR susceptibility antimicrobial range. For propose fifty-fecal-swab samples collected from diarrheic freshly-dead-young-rabbits were presented bacteriological examination. Data showed twenty-seven (54%) gave positive results of Escherichia coli. in-vitro Pathogenicity-test Congo-red(CR) binding assay eight coli isolates (29.63%) Congo-red positive. Antimicrobial several antibiotics was studied. different degrees antibiotics, 3,4 8 categorized as multidrug resistant isolates. Three selected serologically identified classified O169, O125 O158 serotypes for 4,8,3 respectively. Conventional polymerase chain reaction detection eaeA Stx1 revealed expressed gene, but others, O169 don't expressed. While, none gene. Phages may be used effectively control pathogenic colonizing farm rabbit's intestines. However, harsh acidic-conditions digestive enzymes activities influence phage infectivity, decrease efficiency application-trails. Natural-defensive-barrier development being suitable oral administration poultry acid-stability. Encapsulated pahge beads gelatin chitosan -matrix partial titer reductions. Phage titers constant storage water, complete release achieved after 6hr simulated intestinal solution at 37°C. Finding enterohaemorrhagic, created severe health hazard contact human. are promising cost-effective method bacteriophage targeting bacteria farm-rabbits.